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affinity purified rabbit anti cdx2 antibody  (Bethyl)


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    Bethyl affinity purified rabbit anti cdx2 antibody
    Figure 1. Brg1 is required for repression of Oct4 expression at the blastocyst stage. (A) qRT-PCR analysis of Brg1, <t>Cdx2,</t> and Oct4 transcripts in Brg1 KD 8-cell embryos, morulae, and blastocysts. Data were normalized to Ubtf (house keeping gene) and are relative to control embryos at each stage; black line = 1. Asterisk denotes significant difference between Brg1 KD and control blastocysts (p,0.05). (B) ICC analysis of Oct4 and Cdx2 expression in Brg1 KD 8-cell embryos, morulae, and blastocysts. Nuclei were counter stained with DAPI (blue). (C) Brg1 represses Oct4 expression in a dose dependent manner. One-cell embryos were injected with 0 mM (control), 0.1 mM, 1 mM, or 100 mM Brg1 siRNA and cultured to the blastocyst stage. Real-time qPCR was used to analyze the levels of Brg1 and Oct4 transcripts. Data were normalized to Ubtf and are relative to control blastocysts; dashed line = 1. doi:10.1371/journal.pone.0010622.g001
    Affinity Purified Rabbit Anti Cdx2 Antibody, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/affinity purified rabbit anti cdx2 antibody/product/Bethyl
    Average 93 stars, based on 26 article reviews
    affinity purified rabbit anti cdx2 antibody - by Bioz Stars, 2026-03
    93/100 stars

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    1) Product Images from "Brg1 is required for Cdx2-mediated repression of Oct4 expression in mouse blastocysts."

    Article Title: Brg1 is required for Cdx2-mediated repression of Oct4 expression in mouse blastocysts.

    Journal: PloS one

    doi: 10.1371/journal.pone.0010622

    Figure 1. Brg1 is required for repression of Oct4 expression at the blastocyst stage. (A) qRT-PCR analysis of Brg1, Cdx2, and Oct4 transcripts in Brg1 KD 8-cell embryos, morulae, and blastocysts. Data were normalized to Ubtf (house keeping gene) and are relative to control embryos at each stage; black line = 1. Asterisk denotes significant difference between Brg1 KD and control blastocysts (p,0.05). (B) ICC analysis of Oct4 and Cdx2 expression in Brg1 KD 8-cell embryos, morulae, and blastocysts. Nuclei were counter stained with DAPI (blue). (C) Brg1 represses Oct4 expression in a dose dependent manner. One-cell embryos were injected with 0 mM (control), 0.1 mM, 1 mM, or 100 mM Brg1 siRNA and cultured to the blastocyst stage. Real-time qPCR was used to analyze the levels of Brg1 and Oct4 transcripts. Data were normalized to Ubtf and are relative to control blastocysts; dashed line = 1. doi:10.1371/journal.pone.0010622.g001
    Figure Legend Snippet: Figure 1. Brg1 is required for repression of Oct4 expression at the blastocyst stage. (A) qRT-PCR analysis of Brg1, Cdx2, and Oct4 transcripts in Brg1 KD 8-cell embryos, morulae, and blastocysts. Data were normalized to Ubtf (house keeping gene) and are relative to control embryos at each stage; black line = 1. Asterisk denotes significant difference between Brg1 KD and control blastocysts (p,0.05). (B) ICC analysis of Oct4 and Cdx2 expression in Brg1 KD 8-cell embryos, morulae, and blastocysts. Nuclei were counter stained with DAPI (blue). (C) Brg1 represses Oct4 expression in a dose dependent manner. One-cell embryos were injected with 0 mM (control), 0.1 mM, 1 mM, or 100 mM Brg1 siRNA and cultured to the blastocyst stage. Real-time qPCR was used to analyze the levels of Brg1 and Oct4 transcripts. Data were normalized to Ubtf and are relative to control blastocysts; dashed line = 1. doi:10.1371/journal.pone.0010622.g001

    Techniques Used: Expressing, Quantitative RT-PCR, Control, Staining, Injection, Cell Culture

    Figure 2. Expression and localization of Cdx2 and Oct4 in Brg1 KD blastocysts. (A) ICC analysis of Oct4 and Cdx2 in Brg1 KD and control blastocysts. In control blastocysts (a–d) Oct4 expression (green) is restricted to the ICM and is largely absent in the Cdx2-positive (red) trophectoderm. In contrast, in Brg1 KD blastocysts (e–h) Oct4 is widely expressed in both the ICM and cdx2-positive (yellow) trophectoderm. Arrowheads denote co- expression of Oct4 and Cdx2. Nuclei were counter stained with DAPI (blue). (B) Quantification of the average number of cells in Brg1 KD and control blastocysts expressing Oct4, Cdx2, and Oct4 & Cdx2 (double expression). Asterisks denote statistical significance (p,0.05) between Brg1 KD and control blastocysts. A total of 25 Brg1 KD blastocysts and 15 control blastocysts were analyzed. doi:10.1371/journal.pone.0010622.g002
    Figure Legend Snippet: Figure 2. Expression and localization of Cdx2 and Oct4 in Brg1 KD blastocysts. (A) ICC analysis of Oct4 and Cdx2 in Brg1 KD and control blastocysts. In control blastocysts (a–d) Oct4 expression (green) is restricted to the ICM and is largely absent in the Cdx2-positive (red) trophectoderm. In contrast, in Brg1 KD blastocysts (e–h) Oct4 is widely expressed in both the ICM and cdx2-positive (yellow) trophectoderm. Arrowheads denote co- expression of Oct4 and Cdx2. Nuclei were counter stained with DAPI (blue). (B) Quantification of the average number of cells in Brg1 KD and control blastocysts expressing Oct4, Cdx2, and Oct4 & Cdx2 (double expression). Asterisks denote statistical significance (p,0.05) between Brg1 KD and control blastocysts. A total of 25 Brg1 KD blastocysts and 15 control blastocysts were analyzed. doi:10.1371/journal.pone.0010622.g002

    Techniques Used: Expressing, Control, Staining

    Figure 5. Model for Brg1/Cdx2-mediated repression of Oct4 expression in trophectoderm. Schematic diagram of Oct4 regulation in blastocysts. In the trophectoderm Brg1 is recruited to the ARE of the Oct4 promoter via Cdx2. Once at the Oct4 promoter Brg1 and Cdx2 facilitate recruitment of additional co-repressors to repress transcription. doi:10.1371/journal.pone.0010622.g005
    Figure Legend Snippet: Figure 5. Model for Brg1/Cdx2-mediated repression of Oct4 expression in trophectoderm. Schematic diagram of Oct4 regulation in blastocysts. In the trophectoderm Brg1 is recruited to the ARE of the Oct4 promoter via Cdx2. Once at the Oct4 promoter Brg1 and Cdx2 facilitate recruitment of additional co-repressors to repress transcription. doi:10.1371/journal.pone.0010622.g005

    Techniques Used: Expressing



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    affinity purified rabbit anti cdx2 antibody  (Bethyl)
    93
    Bethyl affinity purified rabbit anti cdx2 antibody
    Figure 1. Brg1 is required for repression of Oct4 expression at the blastocyst stage. (A) qRT-PCR analysis of Brg1, <t>Cdx2,</t> and Oct4 transcripts in Brg1 KD 8-cell embryos, morulae, and blastocysts. Data were normalized to Ubtf (house keeping gene) and are relative to control embryos at each stage; black line = 1. Asterisk denotes significant difference between Brg1 KD and control blastocysts (p,0.05). (B) ICC analysis of Oct4 and Cdx2 expression in Brg1 KD 8-cell embryos, morulae, and blastocysts. Nuclei were counter stained with DAPI (blue). (C) Brg1 represses Oct4 expression in a dose dependent manner. One-cell embryos were injected with 0 mM (control), 0.1 mM, 1 mM, or 100 mM Brg1 siRNA and cultured to the blastocyst stage. Real-time qPCR was used to analyze the levels of Brg1 and Oct4 transcripts. Data were normalized to Ubtf and are relative to control blastocysts; dashed line = 1. doi:10.1371/journal.pone.0010622.g001
    Affinity Purified Rabbit Anti Cdx2 Antibody, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/affinity purified rabbit anti cdx2 antibody/product/Bethyl
    Average 93 stars, based on 1 article reviews
    affinity purified rabbit anti cdx2 antibody - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 1. Brg1 is required for repression of Oct4 expression at the blastocyst stage. (A) qRT-PCR analysis of Brg1, Cdx2, and Oct4 transcripts in Brg1 KD 8-cell embryos, morulae, and blastocysts. Data were normalized to Ubtf (house keeping gene) and are relative to control embryos at each stage; black line = 1. Asterisk denotes significant difference between Brg1 KD and control blastocysts (p,0.05). (B) ICC analysis of Oct4 and Cdx2 expression in Brg1 KD 8-cell embryos, morulae, and blastocysts. Nuclei were counter stained with DAPI (blue). (C) Brg1 represses Oct4 expression in a dose dependent manner. One-cell embryos were injected with 0 mM (control), 0.1 mM, 1 mM, or 100 mM Brg1 siRNA and cultured to the blastocyst stage. Real-time qPCR was used to analyze the levels of Brg1 and Oct4 transcripts. Data were normalized to Ubtf and are relative to control blastocysts; dashed line = 1. doi:10.1371/journal.pone.0010622.g001

    Journal: PloS one

    Article Title: Brg1 is required for Cdx2-mediated repression of Oct4 expression in mouse blastocysts.

    doi: 10.1371/journal.pone.0010622

    Figure Lengend Snippet: Figure 1. Brg1 is required for repression of Oct4 expression at the blastocyst stage. (A) qRT-PCR analysis of Brg1, Cdx2, and Oct4 transcripts in Brg1 KD 8-cell embryos, morulae, and blastocysts. Data were normalized to Ubtf (house keeping gene) and are relative to control embryos at each stage; black line = 1. Asterisk denotes significant difference between Brg1 KD and control blastocysts (p,0.05). (B) ICC analysis of Oct4 and Cdx2 expression in Brg1 KD 8-cell embryos, morulae, and blastocysts. Nuclei were counter stained with DAPI (blue). (C) Brg1 represses Oct4 expression in a dose dependent manner. One-cell embryos were injected with 0 mM (control), 0.1 mM, 1 mM, or 100 mM Brg1 siRNA and cultured to the blastocyst stage. Real-time qPCR was used to analyze the levels of Brg1 and Oct4 transcripts. Data were normalized to Ubtf and are relative to control blastocysts; dashed line = 1. doi:10.1371/journal.pone.0010622.g001

    Article Snippet: CDX2 was detected by Western blot analysis using an affinity purified rabbit anti-CDX2 antibody (A300-692A, Bethyl Laboratories, Inc).

    Techniques: Expressing, Quantitative RT-PCR, Control, Staining, Injection, Cell Culture

    Figure 2. Expression and localization of Cdx2 and Oct4 in Brg1 KD blastocysts. (A) ICC analysis of Oct4 and Cdx2 in Brg1 KD and control blastocysts. In control blastocysts (a–d) Oct4 expression (green) is restricted to the ICM and is largely absent in the Cdx2-positive (red) trophectoderm. In contrast, in Brg1 KD blastocysts (e–h) Oct4 is widely expressed in both the ICM and cdx2-positive (yellow) trophectoderm. Arrowheads denote co- expression of Oct4 and Cdx2. Nuclei were counter stained with DAPI (blue). (B) Quantification of the average number of cells in Brg1 KD and control blastocysts expressing Oct4, Cdx2, and Oct4 & Cdx2 (double expression). Asterisks denote statistical significance (p,0.05) between Brg1 KD and control blastocysts. A total of 25 Brg1 KD blastocysts and 15 control blastocysts were analyzed. doi:10.1371/journal.pone.0010622.g002

    Journal: PloS one

    Article Title: Brg1 is required for Cdx2-mediated repression of Oct4 expression in mouse blastocysts.

    doi: 10.1371/journal.pone.0010622

    Figure Lengend Snippet: Figure 2. Expression and localization of Cdx2 and Oct4 in Brg1 KD blastocysts. (A) ICC analysis of Oct4 and Cdx2 in Brg1 KD and control blastocysts. In control blastocysts (a–d) Oct4 expression (green) is restricted to the ICM and is largely absent in the Cdx2-positive (red) trophectoderm. In contrast, in Brg1 KD blastocysts (e–h) Oct4 is widely expressed in both the ICM and cdx2-positive (yellow) trophectoderm. Arrowheads denote co- expression of Oct4 and Cdx2. Nuclei were counter stained with DAPI (blue). (B) Quantification of the average number of cells in Brg1 KD and control blastocysts expressing Oct4, Cdx2, and Oct4 & Cdx2 (double expression). Asterisks denote statistical significance (p,0.05) between Brg1 KD and control blastocysts. A total of 25 Brg1 KD blastocysts and 15 control blastocysts were analyzed. doi:10.1371/journal.pone.0010622.g002

    Article Snippet: CDX2 was detected by Western blot analysis using an affinity purified rabbit anti-CDX2 antibody (A300-692A, Bethyl Laboratories, Inc).

    Techniques: Expressing, Control, Staining

    Figure 5. Model for Brg1/Cdx2-mediated repression of Oct4 expression in trophectoderm. Schematic diagram of Oct4 regulation in blastocysts. In the trophectoderm Brg1 is recruited to the ARE of the Oct4 promoter via Cdx2. Once at the Oct4 promoter Brg1 and Cdx2 facilitate recruitment of additional co-repressors to repress transcription. doi:10.1371/journal.pone.0010622.g005

    Journal: PloS one

    Article Title: Brg1 is required for Cdx2-mediated repression of Oct4 expression in mouse blastocysts.

    doi: 10.1371/journal.pone.0010622

    Figure Lengend Snippet: Figure 5. Model for Brg1/Cdx2-mediated repression of Oct4 expression in trophectoderm. Schematic diagram of Oct4 regulation in blastocysts. In the trophectoderm Brg1 is recruited to the ARE of the Oct4 promoter via Cdx2. Once at the Oct4 promoter Brg1 and Cdx2 facilitate recruitment of additional co-repressors to repress transcription. doi:10.1371/journal.pone.0010622.g005

    Article Snippet: CDX2 was detected by Western blot analysis using an affinity purified rabbit anti-CDX2 antibody (A300-692A, Bethyl Laboratories, Inc).

    Techniques: Expressing